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Literature summary extracted from

  • Arnesen, T.; Starheim, K.K.; Van Damme, P.; Evjenth, R.; Dinh, H.; Betts, M.J.; Ryningen, A.; Vandekerckhove, J.; Gevaert, K.; Anderson, D.
    The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation (2010), Mol. Cell. Biol., 30, 1898-1909.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.255 cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.255 cytosol
-
 Homo sapiens 5829
-
2.3.1.255 ribosome the HYPK-hNatA interaction may occur at the ribosome, active translation is not obligatory for the interaction to occur. Thus, HYPK stably associates with hNatA, common binding to the ribosomes Homo sapiens 5840
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.255 acetyl-CoA + PCNP protein Homo sapiens i.e. PEST proteolytic signal-containing nuclear protein CoA + Nalpha-acetyl-PCNP protein
-
 ?
2.3.1.255 acetyl-CoA + peptide Homo sapiens
-
 CoA + Nalpha-acetylpeptide
-
 ?
2.3.1.255 additional information Homo sapiens endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.255 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.255 recombinant His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p from Escherichia coli by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.255 HEK-293 cell
-
 Homo sapiens
-
2.3.1.255 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.255 acetyl-CoA + ACTH peptide 17 amino acids are identical to the adrenocorticotropin (ACTH) peptide sequence, the ACTH-derived lysines are replaced by arginines to minimize any potential interference by Nalpha-acetylation Homo sapiens CoA + ?
-
 ?
2.3.1.255 acetyl-CoA + PCNP protein i.e. PEST proteolytic signal-containing nuclear protein Homo sapiens CoA + Nalpha-acetyl-PCNP protein
-
 ?
2.3.1.255 acetyl-CoA + peptide
-
 Homo sapiens CoA + Nalpha-acetylpeptide
-
 ?
2.3.1.255 acetyl-CoA + SESSSKSRWGRPVGRRRRPVRVYP high-mobility-group protein A1 sequence Homo sapiens CoA + Ac-SESSSKSRWGRPVGRRRRPVRVYP
-
 ?
2.3.1.255 additional information endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.255 NatA
-
 Homo sapiens
2.3.1.255 NatA protein Nalpha-terminal-acetyltransferase complex
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.255 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.255 8.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.255 acetyl-CoA
-
 Homo sapiens